[抗凝剂对密度梯度离心法分离淋巴细胞的影响]
[Effect of anticoagulants on lymphocyte isolation by density gradient centrifugation].
作者信息
Gnojkowski J, Klimek D, Chmiel J, Tabaka K
出版信息
Acta Haematol Pol. 1981 Apr-Jun;12(2):77-83.
PMID:7304089
Abstract
The authors compared the efficiency of isolation and the purity of lymphocytes obtained by the method of centrifugation in a gradient of ficoll and uropoline from blood obtained on ACD fluid and on heparin. Isolation of lymphocytes from the blood obtained on heparin was more efficient (60%) and useful. For removal of platelets it was necessary to use additional centrifugation in a saccharose gradient or application of aggregating agents during isolation of lymphocytes obtained on heparin or ACD fluid.
摘要
作者比较了通过在菲可和尿嘧啶梯度中离心法从用ACD液和肝素采集的血液中分离淋巴细胞的效率及所获淋巴细胞的纯度。从用肝素采集的血液中分离淋巴细胞效率更高（60%）且更有效。为去除血小板，在从用肝素或ACD液采集的血液中分离淋巴细胞时，有必要在蔗糖梯度中进行额外离心或使用聚集剂。
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