一种通过蔗糖从淋巴细胞 - 血小板混合物中去除血小板的简单方法。
A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose.
作者信息
Chanarat P, Chiewsilp P
出版信息
Am J Clin Pathol. 1975 Feb;63(2):237-9. doi: 10.1093/ajcp/63.2.237.
PMID:803775
Abstract
A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relatively low contamination with erythrocytes and platelets.
摘要
本文介绍了一种通过Ficoll-Hypaque密度离心从人血中分离淋巴细胞的方法，该方法使用密度为65%的单一蔗糖溶液去除血小板。Ficoll-Hypaque-蔗糖法可获得良好的淋巴细胞产量、高活力和纯度，且红细胞和血小板污染相对较低。
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