Makoff A J, Malcolm A D
Biochem J. 1981 Jan 1;193(1):245-9. doi: 10.1042/bj1930245.
The rate of hydrolysis of the imido ester methyl acetimidate and its rate of amidination of denatured aldolase were investigated under different conditions of temperature, pH and ionic strength. Both rate constants increase greatly with temperature, whereas ionic strength has no effect on either. The effect of pH is more complex. Between pH 6.8 and 8.8 the rate of hydrolysis decreases and the rate of amidination increases. These results are discussed in terms of the reaction mechanisms involved.
在不同温度、pH值和离子强度条件下，研究了亚氨酯甲基乙酰亚胺的水解速率及其对变性醛缩酶的脒化速率。两个速率常数均随温度大幅增加，而离子强度对两者均无影响。pH值的影响更为复杂。在pH 6.8至8.8之间，水解速率降低，脒化速率增加。根据所涉及的反应机理对这些结果进行了讨论。
