胰蛋白酶和胰蛋白酶原的钙结合常数。重新评估。
Calcium-binding constants of trypsin and trypsinogen. A reassessment.
作者信息
Cliffe S G, Grant D A
出版信息
Biochem J. 1981 Feb 1;193(2):655-8. doi: 10.1042/bj1930655.
Abstract
The Ca2+-binding constants for trypsin and trypsinogen have been reassessed by using enzyme that has been purified by affinity chromatography and measuring the distribution of 45Ca2+ between the protein and a cation exchanger. The pKCa2+ value of 4.5 for the high-affinity site on trypsin was 1 logarithmic unit greater than that previously reported.
摘要
通过使用经亲和层析纯化的胰蛋白酶，并测量45Ca2+在蛋白质和阳离子交换剂之间的分布，重新评估了胰蛋白酶和胰蛋白酶原的Ca2+结合常数。胰蛋白酶高亲和力位点的pKCa2+值为4.5，比先前报道的值高1个对数单位。
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本文引用的文献
1
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J Biol Chem. 1950 Jul;185(1):387-98.
2
On the activation of trypsinogen. A study of peptide models related to the N-terminal sequence of the zymogen.
Biochem Biophys Res Commun. 1967 Oct 26;29(2):235-40. doi: 10.1016/0006-291x(67)90593-1.
3
The binding of Ca2+ to trypsinogen and its relation to the mechanism of activation.
Biochem Biophys Res Commun. 1967 Aug 7;28(3):390-4. doi: 10.1016/0006-291x(67)90323-3.
4
The mechanism of activation of trypsinogen. The role of the four N-terminal aspartyl residues.胰蛋白酶原的激活机制。四个N端天冬氨酸残基的作用。
Eur J Biochem. 1969 Apr;8(3):314-24. doi: 10.1111/j.1432-1033.1969.tb00530.x.
5
Determination of the operational molarity of solutions of bovine alpha-chymotrypsin, trypsin, thrombin and factor Xa by spectrofluorimetric titration.通过荧光光谱滴定法测定牛α-糜蛋白酶、胰蛋白酶、凝血酶和Xa因子溶液的操作摩尔浓度。
Biochem J. 1973 Jan;131(1):107-17. doi: 10.1042/bj1310107.
6
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Arch Biochem Biophys. 1973 Feb;154(2):501-9. doi: 10.1016/0003-9861(73)90002-7.
7
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8
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