Gevers G, Stalmans W
Biochem J. 1981 Mar 1;193(3):793-8. doi: 10.1042/bj1930793.
Two radiochemical procedures were explored for the determination of phosphorylase activity in the glycogenolytic direction. In the "32P assay method' the formation of labelled glucose 1-phosphate from glycogen and [32P]Pi is measured by the radio-activity that remains soluble after the precipitation of phosphomolybdate with triethylamine. In the "14C assay method' the formation of labelled glucose 1-phosphate from peripherally 14C-labelled glycogen and P1 is determined from the radioactivity that remains soluble after the precipitation of glycogen with ethanol. The 14C assay method requires more preparative work but less circumspection than does the 32P assay method. Both radiochemical methods can be applied where the classical spectrophotometric assay fails. They have the same accuracy and reproducibility, and allow more samples to be handled in parallel. They are not intended for use with crude tissue extracts.
探索了两种放射化学方法来测定糖原分解方向的磷酸化酶活性。在“³²P 测定法”中，通过用三乙胺沉淀磷钼酸盐后仍可溶的放射性来测量由糖原和[³²P]Pi 形成标记的葡萄糖 1-磷酸。在“¹⁴C 测定法”中，由外周¹⁴C 标记的糖原和 P₁形成标记的葡萄糖 1-磷酸是根据用乙醇沉淀糖原后仍可溶的放射性来确定的。¹⁴C 测定法比³²P 测定法需要更多的准备工作，但所需的谨慎程度较低。当经典分光光度测定法不适用时，两种放射化学方法都可以应用。它们具有相同的准确性和重现性，并且允许并行处理更多样品。它们不适用于粗组织提取物。
