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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Clarke N G, Dawson R M

Biochem J. 1981 Apr 1;195(1):301-6. doi: 10.1042/bj1950301.

Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O leads to N-transacylation with N-methyl fatty acid amides being formed. 2. The reagent can be removed easily by volatilization and under defined conditions no secondary decomposition of the phosphorus-containing deacylation products occurs. 3. The water-soluble phosphorus compounds derived by deacylation of mammalian tissue O-diacylated phospholipids have been completely separated by a single-dimensional paper ionophoresis with a volatile pH9 buffer. 4. The O-deacylated alkyl and alkenyl phospholipids have been examined by t.l.c. before and after catalytic hydrolysis with Hg2+. 5. A complete analysis of rat brain phospholipids by the above methods agrees closely with that obtained by other procedures.

通过与含有甲胺、甲醇和水的试剂孵育，实现了磷脂的定量O-脱酰基化。该反应主要是O到N的转酰基化反应，生成N-甲基脂肪酸酰胺。2. 该试剂可通过挥发轻松去除，在规定条件下，含磷脱酰基产物不会发生二次分解。3. 通过使用挥发性pH9缓冲液的单向纸离子电泳，已将哺乳动物组织O-二酰化磷脂脱酰基衍生的水溶性磷化合物完全分离。4. 在用Hg2+催化水解之前和之后，通过薄层层析法对O-脱酰基化的烷基和烯基磷脂进行了检测。5. 通过上述方法对大鼠脑磷脂进行的完整分析与通过其他方法获得的结果非常吻合。

Clarke N G, Dawson R M

Biochem J. 1981 Apr 1;195(1):301-6. doi: 10.1042/bj1950301.

Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O leads to N-transacylation with N-methyl fatty acid amides being formed. 2. The reagent can be removed easily by volatilization and under defined conditions no secondary decomposition of the phosphorus-containing deacylation products occurs. 3. The water-soluble phosphorus compounds derived by deacylation of mammalian tissue O-diacylated phospholipids have been completely separated by a single-dimensional paper ionophoresis with a volatile pH9 buffer. 4. The O-deacylated alkyl and alkenyl phospholipids have been examined by t.l.c. before and after catalytic hydrolysis with Hg2+. 5. A complete analysis of rat brain phospholipids by the above methods agrees closely with that obtained by other procedures.

通过与含有甲胺、甲醇和水的试剂孵育，实现了磷脂的定量O-脱酰基化。该反应主要是O到N的转酰基化反应，生成N-甲基脂肪酸酰胺。2. 该试剂可通过挥发轻松去除，在规定条件下，含磷脱酰基产物不会发生二次分解。3. 通过使用挥发性pH9缓冲液的单向纸离子电泳，已将哺乳动物组织O-二酰化磷脂脱酰基衍生的水溶性磷化合物完全分离。4. 在用Hg2+催化水解之前和之后，通过薄层层析法对O-脱酰基化的烷基和烯基磷脂进行了检测。5. 通过上述方法对大鼠脑磷脂进行的完整分析与通过其他方法获得的结果非常吻合。