肺炎球菌I-192R（美国典型培养物保藏中心12213号）的磷脂。在温和条件下发生的一些结构重排。

The phospholipids of Pneumococcus I-192R, A.T.C.C. 12213. Some structural rearrangements occurring under mild conditions.

Brundish D E, Shaw N, Baddiley J

Biochem J. 1967 Jul;104(1):205-11. doi: 10.1042/bj1040205.

Abstract

The phospholipids from the non-capsulated strain of Pneumococcus I-192R, A.T.C.C. 12213, were separated into three fractions by chromatography on columns of silicic acid and DEAE-cellulose (acetate form). 2. The water-soluble phosphate esters produced by deacylation of each fraction were separated by chromatography on columns of DEAE-cellulose (HCO(3) (-) form). 3. Three deacylated products, diglycerol phosphate, glycerylphosphorylglycerol phosphate and bis(glycerylphosphoryl)glycerol, were identified by analysis, by chemical degradations and by comparison with synthetic materials. 4. From a study of freshly isolated lipids prepared and worked up under conditions where exposure to acid was minimal, it was concluded that the Pneumococcus contains phosphatidylglycerol and bisphosphatidylglycerol, in the molar proportion 1:2.5-3.0, and that the deacylation product glycerylphosphorylglycerol phosphate was probably an artifact of the isolation procedure. 5. Acid-catalysed isomerization (phosphodiester migration) of diglycerol phosphate and bis(glycerylphosphoryl)glycerol and transesterification (glycerol phosphate transfer) of diglycerol phosphate were observed. The structures of the products were established by degradation. 6. A novel mechanism for the biosynthesis of bisphosphatidylglycerol is presented.

摘要

将来自肺炎球菌I - 192R非荚膜菌株（美国典型培养物保藏中心12213）的磷脂通过在硅酸柱和DEAE - 纤维素（醋酸盐形式）柱上进行色谱分离，分成三个组分。2. 各组分脱酰基产生的水溶性磷酸酯通过在DEAE - 纤维素（HCO₃⁻形式）柱上进行色谱分离。3. 通过分析、化学降解以及与合成材料比较，鉴定出三种脱酰基产物，即磷酸二甘油、甘油磷酰甘油磷酸和双（甘油磷酰）甘油。4. 通过对在酸暴露最小的条件下制备和处理的新鲜分离脂质的研究得出结论，肺炎球菌含有磷脂酰甘油和双磷脂酰甘油，摩尔比例为1:2.5 - 3.0，并且脱酰基产物甘油磷酰甘油磷酸可能是分离过程中的人为产物。5. 观察到磷酸二甘油和双（甘油磷酰）甘油的酸催化异构化（磷酸二酯迁移）以及磷酸二甘油的酯交换反应（甘油磷酸转移）。通过降解确定了产物的结构。6. 提出了一种双磷脂酰甘油生物合成的新机制。