Lewis C M, Pegrum G D
Lancet. 1977 Dec 3;2(8049):1151-3. doi: 10.1016/s0140-6736(77)91539-2.
A fluorescein-labelled anti-human immunoglobulin was used to demonstrate that peripheral blood from patients with myelofibrosis had a high proportion of phagocytic cells containing fluorescent immune complexes. Cells from patients with other myeloproliferative diseases (either chronic myeloid leukaemia or polycythaemia rubra vera) did not show similar intracellular immune complexes. Serum from patients with myelofibrosis incubated with polymorphs from healthy subjects caused the appearance of inclusions similar to those found when the patients' own cells were used, the healthy phagocytes apparently engulfing complexes from the patients' sera. The presence of platelets or complement did not alter the incidence of intracellular fluorescence. These tests may help in the diagnosis of myelofibrosis and may also be valuable in recognising the onset of this condition in patients with polycythaemia rubra vera.
使用荧光素标记的抗人免疫球蛋白来证明，骨髓纤维化患者的外周血中有高比例的吞噬细胞含有荧光免疫复合物。患有其他骨髓增殖性疾病（慢性粒细胞白血病或真性红细胞增多症）的患者细胞未显示出类似的细胞内免疫复合物。骨髓纤维化患者的血清与健康受试者的多形核白细胞孵育后，会出现与使用患者自身细胞时发现的类似包涵体，健康的吞噬细胞显然吞噬了患者血清中的复合物。血小板或补体的存在并未改变细胞内荧光的发生率。这些检测可能有助于骨髓纤维化的诊断，在识别真性红细胞增多症患者中这种疾病的发作方面也可能有价值。
