Baglin T P, Price S M, Boughton B J
Department of Haematology, Queen Elizabeth Medical Centre, Edgbaston, Birmingham.
J Clin Pathol. 1990 Feb;43(2):102-5. doi: 10.1136/jcp.43.2.102.
The plasma of patients with myeloproliferative diseases was examined by polyethylene glycol (PEG) precipitation, analytical ultracentrifugation, and immunoaffinity chromatography for the presence of high molecular weight complexes of IgG and fibronectin. Abnormal circulating high molecular weight material was identified by ultracentrifugation in all patients. This was precipitated by PEG and was shown by exclusion chromatography to contain IgG in a high molecular weight form. Examination of plasma by immunoaffinity chromatography supported previous evidence for complex formation between IgG and fibronectin. These findings are further evidence that abnormal high molecular weight IgG complexes are a prominent feature of myeloproliferative disorders and implicate IgG fibronectin complex formation.
采用聚乙二醇（PEG）沉淀法、分析超速离心法和免疫亲和色谱法检测骨髓增殖性疾病患者血浆中是否存在IgG和纤连蛋白的高分子量复合物。通过超速离心法在所有患者中均鉴定出异常循环高分子量物质。该物质可被PEG沉淀，排阻色谱显示其含有高分子量形式的IgG。免疫亲和色谱法检测血浆支持了先前关于IgG与纤连蛋白之间形成复合物的证据。这些发现进一步证明，异常高分子量IgG复合物是骨髓增殖性疾病的一个突出特征，提示IgG纤连蛋白复合物的形成。
