[聚乙二醇用于将外源蛋白显微注射到培养的鼠细胞中]
[Use of polyethylene glycol for the microinjection of exogenous protein into cultured murine cells].
作者信息
Karavanov A A, Chernikov V G, Afanas'ev B N, Liashko V N
出版信息
Ontogenez. 1981;12(4):418-21.
PMID:7306301
Abstract
Polyethylene glycol was applied to microinject two exogenous proteins: bovine serum albumin and non-histone protein derived from mouse spleen chromatin, into the mouse L-cells. The effectiveness of fusion of mammal (human, in the given case) erythrocytes in which hemoglobin is substituted for the protein under study was shown to be higher than when Sendai virus was used. The microinjected proteins preserve their specificity to subcellular structures.
摘要
将聚乙二醇用于向小鼠L细胞显微注射两种外源蛋白质：牛血清白蛋白和源自小鼠脾脏染色质的非组蛋白。结果表明，在以血红蛋白替代所研究蛋白质的哺乳动物（在给定情况下为人类）红细胞融合中，其融合效果高于使用仙台病毒时。显微注射的蛋白质保留了它们对亚细胞结构的特异性。
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