Beljaars P R, Rondags T M
J Assoc Off Anal Chem. 1978 Nov;61(6):1415-8.
A spectrodensitometric method has been developed for the quantitative determination of chloramine-T (N-chloro-N-sodium-p-toluenesulfonamide) in ice cream. Chloramine-T is extracted and converted into p-toluenesulfonamide (p-TSA) followed by thin layer chromatographic separation of concentrated extracts on silica gel and quantitation of the p-TSA spots from standards and samples by direct scanning with a reflectance densitometer at 228 nm. A linear relationship was obtained between recorded peak area and concentration for 0.5--7.0 microgram p-TSA/spot. The reproducibility of the complete method was 2.87% (n = 9 determinations). The detection limit of the scanning procedure was 0.5 microgram p-TSA/spot, corresponding to a concentration of 4 mg chloramine-T/kg sample. The average recovery was 88 +/- 3% (P = 95%) for 10 ice cream samples spiked with chloramine-T at levels ranging from 10 to 55 mg/kg. The described method was used to assay 146 commercial ice cream and whipped cream samples for chloramine-T.
已开发出一种分光密度测定法，用于定量测定冰淇淋中的氯胺 -T（N -氯 -N -对甲苯磺酸钠）。氯胺 -T经提取并转化为对甲苯磺酰胺（p -TSA），然后将浓缩提取物在硅胶上进行薄层色谱分离，并用反射密度计在228nm处直接扫描对标准品和样品中的p -TSA斑点进行定量。对于0.5 - 7.0微克p -TSA/斑点，记录的峰面积与浓度之间呈线性关系。整个方法的重现性为2.87%（n = 9次测定）。扫描程序的检测限为0.5微克p -TSA/斑点，相当于4毫克氯胺 -T/千克样品的浓度。对于10个添加了浓度范围为10至55毫克/千克氯胺 -T的冰淇淋样品，平均回收率为88±3%（P = 95%）。所描述的方法用于分析146个市售冰淇淋和鲜奶油样品中的氯胺 -T。
