碱性化合物对网格蛋白聚合的影响。

Effect of basic compounds on the polymerization of clathrin.

作者信息

Nandi P K, Van Jaarsveld P P, Lippoldt R E, Edelhoch H

出版信息

Biochemistry. 1981 Nov 10;20(23):6706-10. doi: 10.1021/bi00526a028.

Abstract

The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amines had an important effect. Of the several basic proteins evaluated, only lysozyme stimulated the rate of polymerization. Some of these substances were able to increase the rate sufficiently so that polymerization occurred at physiological pH values. Without these compounds, clathrin will only polymerize at pH values of 6.8 or less.

摘要

通过比浊法评估了几种二价阳离子、各种多元碱胺和溶菌酶对8S网格蛋白聚合成300S包被结构速率的影响。Ca2+和Mn2+强烈提高聚合速率。在天然存在的多元碱胺中，只有精胺有重要影响。在所评估的几种碱性蛋白质中，只有溶菌酶刺激聚合速率。其中一些物质能够充分提高速率，从而使聚合在生理pH值下发生。没有这些化合物，网格蛋白仅在pH值为6.8或更低时聚合。