Coppola E D, Conrad E C, Cotter R
J Assoc Off Anal Chem. 1978 Nov;61(6):1490-2.
A reverse phase high pressure liquid chromatographic method is presented for the simultaneous separation and determination of quinic, malic, and citric acids in single strength, undiluted cranberry juice. After a 1 : 10 dilution and cleanup through a disposable column, major organic acids in cranberry juice are separated on a Bondapak/C18 column and quantitated by using a differential refractometer. Twenty-seven samples of different single strength cranberry juice were analyzed using this method; the mean content of quinic, malic, and citric acids were 1.32 (std dev. 0.150), 0.92 (std dev. 0.079), and 1.08% (std dev. 0.111), respectively. Mean percent recoveries of each acid were quinic 95.4 (std dev. 6.8), malic 96.6 (std dev. 5.8), and citric 94.0% (std dev. 4.8).
本文介绍了一种反相高效液相色谱法，用于同时分离和测定单一浓度、未稀释的蔓越莓汁中的奎宁酸、苹果酸和柠檬酸。将蔓越莓汁按1:10稀释后，通过一次性柱进行净化，然后在Bondapak/C18柱上分离蔓越莓汁中的主要有机酸，并使用示差折光仪进行定量。使用该方法分析了27个不同单一浓度的蔓越莓汁样品；奎宁酸、苹果酸和柠檬酸的平均含量分别为1.32（标准差0.150）、0.92（标准差0.079）和1.08%（标准差0.111）。每种酸的平均回收率分别为：奎宁酸95.4（标准差6.8）、苹果酸96.6（标准差5.8）和柠檬酸94.0%（标准差4.8）。
