Stange E F, Schneider A, Preclik G, Ditschuneit H
Biochim Biophys Acta. 1981 Nov 23;666(2):291-3. doi: 10.1016/0005-2760(81)90119-3.
The effect of bile acids and bile acid/cholesterol micelles on 3-hydroxy-3-methylglutaryl coenzyme A reductase, the key enzyme of cholesterol synthesis, was investigated in cultured intestine. Glycocholic and glycodeoxycholic acid both suppressed total (fully activated) reductase activity after 3 h culture. The portion of expressed reductase, determined in the presence of NaF, was unaffected at 3 h, but decreased after 24 h of bile acid treatment. In contrast, total enzyme activity was stimulated up to 2.5-fold at 24 h; this bile acid effect was blocked by additional cholesterol. These results suggest that bile acids modulate both total reductase activity and the activation state of the enzyme in cultured intestine.
在培养的肠道中研究了胆汁酸和胆汁酸/胆固醇胶束对胆固醇合成关键酶3-羟基-3-甲基戊二酰辅酶A还原酶的影响。甘胆酸和甘氨脱氧胆酸在培养3小时后均抑制了总（完全激活）还原酶活性。在氟化钠存在下测定的表达还原酶部分在3小时时未受影响，但在胆汁酸处理24小时后降低。相比之下，总酶活性在24小时时被刺激高达2.5倍；这种胆汁酸效应被额外的胆固醇阻断。这些结果表明胆汁酸在培养的肠道中调节总还原酶活性和酶的激活状态。
