Lancelot G, Mayer R, Thuong N T, Chassignol M, Hélène C
Biochimie. 1981 Oct;63(10):785-90. doi: 10.1016/s0300-9084(81)80038-7.
Proton magnetic resonance spectra of the self-complementary decadeoxynucleotide d(AATTGCAATT) at 90 and 250 MHz have been obtained at different temperatures. The assignment of the different resonance lines to the base protons was obtained by combining the data derived from various methods: hydrogen in equilibrium with deuterium exchange at the H8 position of purines; comparison of NMR spectra obtained at high temperature with those of mononucleotides; comparison of the variations in chemical shifts obtained between 280 K and 360 K with calculated values; determination of half-transition temperatures for each base pair. On the basis of computed chemical shifts for stacked base-pairs it is concluded that the decadeoxynucleotide duplex exists in the B form in solution at 280 K. Propagation of the opening of the mini double helix from terminal to central base pairs if reflected in the variation of half-transition temperatures which vary between 306 K and 327 K.
已在不同温度下获得了自互补十脱氧核苷酸d(AATTGCAATT)在90和250兆赫兹的质子磁共振谱。通过综合各种方法得到的数据，将不同的共振线归属于碱基质子：嘌呤H8位置的氢与氘交换达到平衡；高温下获得的核磁共振谱与单核苷酸的核磁共振谱进行比较；280K至360K之间化学位移的变化与计算值进行比较；确定每个碱基对的半转变温度。根据堆积碱基对的计算化学位移得出结论，十脱氧核苷酸双链体在280K的溶液中以B型存在。微型双螺旋从末端碱基对到中心碱基对的开口扩展反映在半转变温度的变化上，半转变温度在306K至327K之间变化。
