Strzadala L, Opolski A, Radzikowski C, Mihich E
Cancer Res. 1981 Dec;41(12 Pt 1):4934-7.
The different expression of surface antigens on L1210 leukemia DBA/2 and drug-resistant L1210 sublines was investigated. Indirect cytotoxic test, the anti-L1210/v alloantiserum reacted more strongly with subline cells than with parental cells. Absorption of the antiserum with Gross cellular surface antigen-positive AKR leukemia (AKSL-4) cells led to a much greater difference in this reactivity. Quantitative absorption experiments revealed that the drug-resistant sublines had 5 times higher absorption capacity than did the parental line. After complete absorption of antibodies against murine leukemia virus-related antigens, the anti-L1210/v alloantiserum still reacted with L1210 cells. This cytotoxicity could be removed after absorption with C3H mammary tumor (MAC-1) cells but not with normal C3H lymphocytes. These results provide evidence that the major cytotoxic activity of the antiserum against L1210 and L1210 subline cells was due to antibodies against murine mammary tumor virus-related antigen and that the drug-resistant sublines of leukemia L1210 have higher quantitative expression of mammary leukemia antigens.
研究了L1210白血病DBA/2及其耐药亚系表面抗原的不同表达。在间接细胞毒性试验中，抗L1210/v同种异体抗血清与亚系细胞的反应比与亲代细胞的反应更强。用格罗斯细胞表面抗原阳性的AKR白血病（AKSL-4）细胞吸收抗血清后，这种反应性的差异更大。定量吸收实验表明，耐药亚系的吸收能力比亲代系高5倍。在用抗鼠白血病病毒相关抗原的抗体完全吸收后，抗L1210/v同种异体抗血清仍与L1210细胞反应。用C3H乳腺肿瘤（MAC-1）细胞吸收后可消除这种细胞毒性，但用正常C3H淋巴细胞吸收则不能。这些结果证明，抗血清对L1210和L1210亚系细胞的主要细胞毒性活性是由于抗鼠乳腺肿瘤病毒相关抗原的抗体，并且白血病L1210的耐药亚系具有更高的乳腺白血病抗原定量表达。
