Steuden J, Strzadała L, Radzikowski C
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):95-7.
The presence of ML antigen on leukemia L1210 cells was confirmed by different procedures. In addition, by applying appropriate absorptions and blocking techniques combined with ferritin labelled antibodies in electron microscopy (EM), the independent localization of ML antigen and murine leukemia virus Gross (MuLV-Gross) cellular antigen on L1210 cells was demonstrated. The ferritin labeled antigenic sites appeared to be free from budding viral particles. In further studies the 3M KCI extracts of L1210 leukemia cells purified by polyacrylamide gel electrophoresis (PAGE) and the antigenic material from leukemia L1210 cells purified by affinity chromatography were characterized. Their identity was verified by anti ML-sera. Using the modified lyso-strip method, the presence of ML antigen and H-2K and H-2D gene products on independent molecules on the surface of L1210 cells was shown. In addition, in preparative polyacrylamide gel electrophoresis (PAGE) of 3M KCL extracts of L1210 cells, ML and H-2d antigenic products were found in separate fractions.
通过不同程序证实了白血病L1210细胞上存在ML抗原。此外，在电子显微镜（EM）中应用适当的吸收和封闭技术并结合铁蛋白标记抗体，证明了ML抗原和鼠白血病病毒Gross（MuLV-Gross）细胞抗原在L1210细胞上的独立定位。铁蛋白标记的抗原位点似乎没有出芽的病毒颗粒。在进一步的研究中，对通过聚丙烯酰胺凝胶电泳（PAGE）纯化的L1210白血病细胞的3M KCI提取物以及通过亲和层析纯化的白血病L1210细胞的抗原物质进行了表征。用抗ML血清验证了它们的同一性。使用改良的溶菌条法，显示了L1210细胞表面独立分子上存在ML抗原以及H-2K和H-2D基因产物。此外，在L1210细胞的3M KCL提取物的制备性聚丙烯酰胺凝胶电泳（PAGE）中，ML和H-2d抗原产物存在于不同的组分中。
