Leiendecker-Foster C, Freier E F
Clin Chem. 1981 Dec;27(12):2029-32.
We describe an improved gas-chromatographic method for the simultaneous quantitation of the catecholamine metabolites, homovanillic acid (3-methoxy-4-hydroxyphenylacetic acid) and vanillylmandelic acid (3-methoxy-4-hydroxymandelic acid). Our improvements in the method of Muskiet et al. (Clin. Chem. 23: 863, 1977) include a shorter program time and a longer silylation interval. Recovery and precision data obtained by this improved technique are similar to those of Muskiet et al. Vanillylmandelic acid results (y) were compared with those by the method of Pisano et al. (Clin. Chim. Acta 7: 285, 1962). The relation is expressed by the equation y = 0.52 + 1.05x (Sy . x = 2.33 mg/24 h and r = 0.997). Results for homovanillic acid (y) were compared with those by the method of Knight and Haymond (Clin. Chem. 23: 2007, 1977); the equation was y = 0.84 + 0.90x (Sy . x = 2.04 and r = 0.97). Retention times are also reported for several phenolic acids and other related compounds found in urine.
我们描述了一种改进的气相色谱法，用于同时定量儿茶酚胺代谢产物高香草酸（3-甲氧基-4-羟基苯乙酸）和香草扁桃酸（3-甲氧基-4-羟基扁桃酸）。我们对Muskiet等人（《临床化学》23: 863, 1977）方法的改进包括更短的程序时间和更长的硅烷化间隔。通过这种改进技术获得的回收率和精密度数据与Muskiet等人的相似。将香草扁桃酸的结果（y）与Pisano等人（《临床化学学报》7: 285, 1962）的方法所得结果进行比较。两者关系由方程y = 0.52 + 1.05x表示（Sy.x = 2.33 mg/24 h且r = 0.997）。将高香草酸的结果（y）与Knight和Haymond（《临床化学》23: 2007, 1977）的方法所得结果进行比较；方程为y = 0.84 + 0.90x（Sy.x = 2.04且r = 0.97）。还报告了尿液中发现的几种酚酸和其他相关化合物的保留时间。
