Adams S, Lucas D R
Curr Eye Res. 1981;1(5):291-9. doi: 10.3109/02713688108999450.
The survival in organ culture of cat and human corneas in two chemically-defined media, T8 and MAB87/3 was compared with that in MEM containing foetal bovine serum. The endothelium was assessed by SEM and vital staining with nitroblue tetrazolium after periods of up to 28 days. The cat endothelial cell population increased considerably in density in all the media and the orderly hexagonal arrangement of the cells was lost. The derangement was greater in the chemically-defined media after periods of storage exceeding 7 days. The human endothelial cell population declined slightly in all the media and the cells retained a relatively orderly arrangement. Survival in the chemically-defined media appeared equal to that in MEM. We conclude: 1) the observed differences between the species need to be considered when cat endothelium is used as an experimental model; 2) chemically-defined media appear to be satisfactory for preservation of human corneas by organ culture for periods of at least 7 days.
将猫和人类角膜在两种化学成分明确的培养基（T8和MAB87/3）中的器官培养存活率与在含有胎牛血清的MEM中的存活率进行了比较。通过扫描电子显微镜（SEM）和用硝基蓝四唑进行活体染色，在长达28天的时间段后对内皮细胞进行评估。在所有培养基中，猫内皮细胞群体的密度显著增加，细胞有序的六边形排列消失。在储存超过7天后，化学成分明确的培养基中的紊乱情况更严重。在所有培养基中，人类内皮细胞群体略有下降，细胞保持相对有序的排列。化学成分明确的培养基中的存活率似乎与MEM中的存活率相当。我们得出结论：1）当将猫内皮细胞用作实验模型时，需要考虑观察到的物种间差异；2）化学成分明确的培养基似乎足以通过器官培养将人类角膜保存至少7天。
