Carrington S D, Grierson I
Graefes Arch Clin Exp Ophthalmol. 1986;224(3):271-7. doi: 10.1007/BF02143068.
The phagocytosis of IgG-coated latex microspheres by the central and peripheral regions of the corneal endothelium in the domestic cat was investigated by organ culture. The endothelium was exposed to 1.357 X 10(7) microspheres/mm2, and counts of ingested microspheres were made by scanning electron microscopy. Initial assessments of phagocytosis over 8-h and 24-h periods of culture gave extremely low levels of incorporation. Therefore, subsequently, a 48-h period of culture was adopted. Microsphere uptake levels per square millimeter over 48 h were as follows: peripheral endothelium 6,021 +/- 3,162; central endothelium 2,000 +/- 1,529; overall 4,011 +/- 3,294. The greater uptake of microspheres in the peripheral cornea was statistically significant (P less than 0.002, paired t-test). The involvement of microvilli in phagocytosis by corneal endothelial cells was proposed.
通过器官培养研究了家猫角膜内皮中央和周边区域对IgG包被的乳胶微球的吞噬作用。将内皮暴露于1.357×10(7)个微球/mm2，通过扫描电子显微镜对摄取的微球进行计数。在8小时和24小时培养期内对吞噬作用的初步评估显示摄取水平极低。因此，随后采用了48小时的培养期。48小时内每平方毫米的微球摄取水平如下：周边内皮6,021±3,162；中央内皮2,000±1,529；总体4,011±3,294。周边角膜中微球摄取量更大具有统计学意义（P<0.002，配对t检验）。提出了微绒毛参与角膜内皮细胞吞噬作用。
