Rutland J, Griffin W, Cole P
Chest. 1981 Dec;80(6 Suppl):865-7. doi: 10.1378/chest.80.6.865.
Human respiratory tract epithelium suitable for measuring ciliary beat frequency (CBF) may be obtained by nasal brushing. We have used such ciliated epithelium in an in vitro preparation to study the effects on CBF of lidocaine hydrochloride administered topically in vivo before brushing or in vitro after brushing. Beat frequency measured after in vivo administration was unchanged compared to control values while incubating ciliated epithelium in vitro in increasing concentrations of lidocaine resulted in dose-related cilioinhibition. Drug concentrations required to depress ciliary activity in vitro were much higher than those occurring in clinical practice.
可通过鼻刷获取适合测量纤毛摆动频率（CBF）的人呼吸道上皮。我们已在体外制剂中使用这种纤毛上皮，以研究在刷检前局部给予体内或刷检后体外给予盐酸利多卡因对CBF的影响。与对照值相比，体内给药后测得的摆动频率没有变化，而将纤毛上皮在体外置于浓度不断增加的利多卡因中孵育会导致剂量相关的纤毛抑制。体外抑制纤毛活动所需的药物浓度远高于临床实践中出现的浓度。
