Selwyn D A, Raphael J H, Lambert D G, Langton J A
University Department of Anaesthesia, Leicester Royal Infirmary.
Br J Anaesth. 1996 Feb;76(2):274-7. doi: 10.1093/bja/76.2.274.
Using human nasal cytological brushings, we have investigated the effects of morphine on ciliary function by measurement of cilia beat frequency in vitro, and we have also determined opioid receptor binding in these specimens. We obtained ciliated samples from seven volunteers, and measured cilia beat frequency using the transmitted light technique during exposure to morphine 10 mumol litre-1 for 4 h. Mean cilia beat frequency of the samples exposed to morphine was 11.1 (95% confidence interval 10.9-11.5) Hz and that of the controls 11.3 (11.1-11.7) Hz. There was no significant effect of morphine on human cilia beat frequency in vitro (MANOVA for repeated measures and nested, F = 0.61, P = 0.66). In a separate study, we obtained nasal brushings from 20 patients and measured the binding of the opioid antagonist tritiated diprenorphine ([3H]DPN). Mean disintegrations per minute (dpm) for total and non-specific binding were 9036 (8105-9967) dpm and 9130 (8054-10206) dpm, respectively. These values did not differ significantly (paired t test, t = 0.22, P = 0.83). We conclude that morphine had no effect on cilia beat frequency in vitro and we were unable to demonstrate any significant numbers of opioid receptors on nasal ciliated epithelium.
我们使用人体鼻腔细胞学刷片，通过体外测量纤毛摆动频率研究了吗啡对纤毛功能的影响，并且还测定了这些样本中的阿片受体结合情况。我们从7名志愿者身上获取了有纤毛的样本，并在暴露于10微摩尔/升吗啡4小时的过程中，使用透射光技术测量纤毛摆动频率。暴露于吗啡的样本的平均纤毛摆动频率为11.1（95%置信区间10.9 - 11.5）赫兹，而对照组为11.3（11.1 - 11.7）赫兹。吗啡在体外对人体纤毛摆动频率没有显著影响（重复测量和嵌套的多变量方差分析，F = 0.61，P = 0.66）。在另一项研究中，我们从20名患者身上获取了鼻腔刷片，并测量了阿片类拮抗剂氚化二丙诺啡（[³H]DPN）的结合情况。总结合和非特异性结合的平均每分钟衰变数（dpm）分别为9036（8105 - 9967）dpm和9130（8054 - 10206）dpm。这些值没有显著差异（配对t检验，t = 0.22，P = 0.83）。我们得出结论，吗啡在体外对纤毛摆动频率没有影响，并且我们无法证明鼻腔纤毛上皮细胞上存在任何大量的阿片受体。
