Batey R G, Shamir S, Wilms J
Dig Dis Sci. 1981 Dec;26(12):1084-8. doi: 10.1007/BF01295972.
These experiments have examined the physical properties and the hepatic uptake of non-transferrin-bound iron, a fraction found in the serum of untreated primary hemochromatosis patients. Ultrafiltration, polyacrylamide gel electrophoresis, and TLC have demonstrated the non-transferrin-bound iron complex to have a molecular weight less than 1000 and to be distinct from free iron and amino acid iron complexes. Hepatic iron uptake from serum containing non-transferrin-bound iron was significantly higher than from the same serum sample from which the abnormal iron had been removed. Non-transferrin-bound iron entering the liver was localized to the lysosomal fraction in the first hour and was subsequently incompletely incorporated into ferritin. The results demonstrated an abnormal uptake of this iron fraction by the liver and suggest that the abnormal serum fraction may contribute to tissue damage by entering the lysosomes.
这些实验研究了非转铁蛋白结合铁的物理性质及其肝脏摄取情况，非转铁蛋白结合铁是未经治疗的原发性血色素沉着症患者血清中发现的一种成分。超滤、聚丙烯酰胺凝胶电泳和薄层色谱法已证明，非转铁蛋白结合铁复合物的分子量小于1000，且与游离铁和氨基酸铁复合物不同。从含有非转铁蛋白结合铁的血清中摄取肝脏铁的量显著高于从去除了异常铁的同一血清样本中摄取的量。进入肝脏的非转铁蛋白结合铁在第一小时定位于溶酶体部分，随后未完全整合到铁蛋白中。结果表明肝脏对这种铁成分的摄取异常，并提示异常血清成分可能通过进入溶酶体而导致组织损伤。
