通过液相色谱法和柱前标记荧光检测法测定多胺

Determination of polyamines by liquid chromatography and precolumn labelling for fluorescence detection.

作者信息

Vandemark F L, Schmidt G J, Slavin W

出版信息

J Chromatogr Sci. 1978 Oct 10;16(10):465-9. doi: 10.1093/chromsci/16.10.465.

DOI:10.1093/chromsci/16.10.465

Abstract

Procedures are described for the separation and detection of picomole quantities of putrescine, spermidine, and spermine by liquid chromatography. The polyamines are labelled by precolumn derivatization with dansyl chloride followed by reversed-phase chromatography with a methanol and water mobile phase. The derivatized polyamines are measured with a fluorescence detector using an excitation wavelength of 340 nm and emission wavelength of 515 nm. The polyamines are eluted within 12 min and 0.5 ng of each could be detected. Some preliminary data on urine samples is presented.

摘要

描述了通过液相色谱法分离和检测皮摩尔量的腐胺、亚精胺和精胺的方法。通过用丹磺酰氯进行柱前衍生化，然后用甲醇和水流动相进行反相色谱法对多胺进行标记。使用荧光检测器，在激发波长为340nm和发射波长为515nm的条件下测量衍生化的多胺。多胺在12分钟内洗脱，每种多胺可检测到0.5ng。还给出了一些尿液样本的初步数据。