通过薄层色谱法估算尿中二胺和多胺的含量。
Estimation of urinary diamines and polyamines by thin-layer chromatography.
作者信息
Fleisher J H, Russell D H
出版信息
J Chromatogr. 1975 Jul 16;110(2):335-40. doi: 10.1016/0021-9673(75)85014-x.
The dansylated derivatives of ammonia, 1,3-diaminopropane, 1,4-diaminobutane, 1,5-diaminopentane, spermidine, histamine, and spermine were separated by one ascending development in chloroform-triethylamine (5:1) on a 250-mum silica gel 60 plate. Putrescine, cadaverine, spermidine, and spermine in human urine were quantitated by a direct scan of the fluorescent intensity of the spots corresponding to these compounds. Higher amounts of spermidine and spermine were found in the urines of cancer patients compared to the values of these substances in normal urine.
氨、1,3 - 二氨基丙烷、1,4 - 二氨基丁烷、1,5 - 二氨基戊烷、亚精胺、组胺和精胺的丹磺酰化衍生物，在250μm硅胶60板上，通过在氯仿 - 三乙胺（5:1）中进行一次上行展开进行分离。通过直接扫描与这些化合物相对应的斑点的荧光强度，对人尿中的腐胺、尸胺、亚精胺和精胺进行定量。与正常尿液中这些物质的值相比，癌症患者尿液中发现的亚精胺和精胺含量更高。
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