Chiraseveenuprapund P, Rosenberg I N
Endocrinology. 1981 Dec;109(6):2095-102. doi: 10.1210/endo-109-6-2095.
In bovine thyroid slices, the inhibition of organic binding of iodide by excess iodide in the range 5-10 micrograms/ml was prevented by incubating the slices in the presence of TSH. The Wolff-Chaikoff effect was also overcome by the presence of a hydrogen peroxide-generating system, such as glucose-glucose-oxidase or tyramine. TSH and hydrogen peroxide enhanced the synthesis of both iodotyrosines and iodothyronines. The enhanced organification of iodine in the presence of TSH or hydrogen peroxide was not due to an abrupt synthesis of organic iodine during the early phase of incubation before intrathyroidal iodide concentrations had reached the inhibitory levels. These findings suggest that the inhibition of organic binding of iodine in the presence of excess iodide may be due to a diminished generation or a decreased availability of hydrogen peroxide in the thyroid.
在牛甲状腺切片中，通过在促甲状腺激素（TSH）存在的情况下孵育切片，可防止5 - 10微克/毫升范围内过量碘对碘化物有机结合的抑制作用。过氧化氢生成系统（如葡萄糖 - 葡萄糖氧化酶或酪胺）的存在也能克服沃尔夫 - 柴科夫效应。TSH和过氧化氢可增强碘酪氨酸和碘甲状腺原氨酸的合成。在TSH或过氧化氢存在下碘有机化增强并非由于在甲状腺内碘化物浓度达到抑制水平之前孵育早期有机碘的突然合成。这些发现表明，在过量碘存在下碘有机结合的抑制可能是由于甲状腺中过氧化氢生成减少或可用性降低所致。
