Komolafe O O
J Chromatogr Sci. 1978 Oct 10;16(10):496-9. doi: 10.1093/chromsci/16.10.496.
Ion-pair partition has been combined with HPLC for the rapid detection and separation of the major anthraquinone glycosides of senns, sennosides A and B. A Corasil C18 column (5 microns) with methyl alcohol (30 percent in water) containing 0.005 M tetrabutylammonium phosphate, pH 7.5, as mobile phase was used. In the application to the assay of drugs, sennosides A and/or B constituents of the crude senna drug, Senokot syrup, Senokot tablets, and Pursennid tablets have been estimated in the presence of other constituents of the crude drug and pharmaceutical preparations. The results obtained are compared with colorimetric assay results.
离子对分配法已与高效液相色谱法相结合，用于快速检测和分离番泻叶中的主要蒽醌苷，即番泻苷A和B。使用了Corasil C18柱（5微米），以含有0.005 M磷酸四丁铵（pH 7.5）的甲醇（30%水）作为流动相。在药物分析应用中，已对粗制番泻叶药物、塞纳科糖浆、塞纳科片剂和番泻叶胶囊中的番泻苷A和/或B成分进行了测定，同时考虑了粗制药物和药物制剂中的其他成分。将所得结果与比色法测定结果进行了比较。
