Abe S I
Differentiation. 1981;20(1):65-70. doi: 10.1111/j.1432-0436.1981.tb01157.x.
Dissociated spermatogenic cells were cultivated within the collagen matrix at low cell density. The largest cell type in the culture was identified as the primary spermatocytes by their size and the morphological characteristics revealed by ultra-thin sections. Chromosome analysis showed that about 90% of the cells examined were either in first or second meiosis. Within the collagen matrix, the fates of 282 single primary spermatocytes at meiotic stage in diakinesis or metaphase were followed. In a few days, most of them gave rise to four spermatids, passing through first and second meiotic divisions. About 80% of the spermatids formed motile flagella. They grew about 20-60 micrometers a day. The final state of the differentiation attained in our culture conditions was the spermatids with localized spherical nuclei and motile flagella, about 500 micrometers in length after 1-month's culture. Ultra-thin sections of the spermatids show that the rings, neck-pieces, and acrosomes developed in the cells.
将生精细胞在胶原基质中以低密度培养。培养物中最大的细胞类型根据其大小和超薄切片显示的形态特征被鉴定为初级精母细胞。染色体分析表明，约90%的检测细胞处于第一次或第二次减数分裂期。在胶原基质中，追踪了282个处于终变期或中期减数分裂阶段的单个初级精母细胞的命运。几天内，它们中的大多数经过第一次和第二次减数分裂产生了四个精子细胞。约80%的精子细胞形成了可运动的鞭毛。它们每天生长约20 - 60微米。在我们的培养条件下达到的最终分化状态是具有局部球形细胞核和可运动鞭毛的精子细胞，培养1个月后长度约为500微米。精子细胞的超薄切片显示细胞中形成了环、颈部和顶体。
