Saiki A, Tamura M, Matsumoto M, Katowgi J, Watanabe A, Onitake K
Department of Biology, Faculty of Science, Yamagata University, Japan.
Dev Growth Differ. 1997 Jun;39(3):337-44. doi: 10.1046/j.1440-169x.1997.t01-2-00009.x.
Spermatocytes of the teleost, Oryzias latipes, at meiotic prophase were cultured without contact with somatic cells. They began to divide, progressing through the meiotic divisions and differentiating into round spermatids within 48 h. The chromosome number in both the primary and secondary spermatocytes at metaphase was n = 24. In spermatids, a single flagellum was formed and the release of residual bodies was observed in vitro. The size and shape of the flagellum were the same as those seen in vivo. The expression of protamine mRNA was detected in round spermatids. This result suggests that gene expression, as well as morphological change, is regulated by the progression of spermatogenesis in cell culture. Furthermore, when the eggs of O. latipes were inseminated with germ cells cultured for 10 days, normal embryos developed and hatched out. These results suggest that the spermatocytes of O. latipes develop into fertile sperm in cell culture.
将硬骨鱼青鳉减数分裂前期的精母细胞在不与体细胞接触的情况下进行培养。它们开始分裂，经历减数分裂并在48小时内分化为圆形精子细胞。中期初级和次级精母细胞的染色体数均为n = 24。在精子细胞中，形成了单一的鞭毛，并在体外观察到残余体的释放。鞭毛的大小和形状与体内观察到的相同。在圆形精子细胞中检测到鱼精蛋白mRNA的表达。这一结果表明，在细胞培养中，基因表达以及形态变化受精子发生进程的调控。此外，当用培养10天的生殖细胞对青鳉卵进行授精时，正常胚胎发育并孵化出来。这些结果表明，青鳉的精母细胞在细胞培养中发育成可育精子。
