蛋白质对肠道细菌共同抗原免疫原性的影响。

Effect of proteins on the immunogenicity of enterobacterial common antigen.

作者信息

Kuhn H M, Adamus G, Romanowska E, Mayer H

出版信息

Infect Immun. 1981 Nov;34(2):373-7. doi: 10.1128/iai.34.2.373-377.1981.

DOI:10.1128/iai.34.2.373-377.1981

PMID:7309231

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC350876/

Abstract

Enterobacterial common antigen isolated by two independent extraction procedures was found to precipitate with a number of basic or hydrophobic proteins. Complexes of enterobacterial common antigen with protamine sulfate, with methylated bovine serum albumin or with a fraction of outer membrane proteins of two different Shigella wild types proved to be highly immunogenic in rabbits upon intravenous immunization, in contrast to the enterobacterial common antigen preparations by themselves. This explains why crude isolates of enterobacterial common antigen usually are good immunogens in contrast to the isolated antigen, which was described to be either not or only very poorly immunogenic.

摘要

通过两种独立提取程序分离得到的肠道细菌共同抗原，被发现能与多种碱性或疏水蛋白发生沉淀反应。肠道细菌共同抗原与硫酸鱼精蛋白、甲基化牛血清白蛋白或两种不同志贺氏菌野生型的一部分外膜蛋白形成的复合物，经静脉注射免疫兔子后，被证明具有高度免疫原性，这与肠道细菌共同抗原制剂本身形成对比。这就解释了为什么肠道细菌共同抗原的粗提物通常是良好的免疫原，而与之形成对比的是，分离出的抗原据描述要么没有免疫原性，要么免疫原性非常差。