Ramia S, Amsterdam D, Mayer H, Neter E
Immunol Commun. 1982;11(6):491-4. doi: 10.3109/08820138209050745.
Pseudomonas aeruginosa produces a factor (PF) which alters the enterobacterial common antigen (ECA). Its effect on the immunogenicity of two types of immunogenic ECA, namely, the ethanol-soluble preparation freed of lipopolysaccharide and the LPS-coupled form from the R-mutant E. coli 014 was investigated. The antibody response following intravenous immunization was determined by means of the hemagglutination test. It is shown that PF abolishes the immunogenicity of the former but not of the latter. PF obtained from a strain of P. maltophilia yielded the same results. Antiserum against Pseudomonas aeruginosa of types 1 and 6 neutralizes PF produced by either type. These results suggest that PF alters the lipid part and not the haptenic determinant of ECA and that this activity is neutralized by P. aeruginosa antiserum of either type 1 or type 6. This interpretation is compatible with the identification of PF as a lipase.
铜绿假单胞菌产生一种因子（PF），它能改变肠道细菌共同抗原（ECA）。研究了该因子对两种免疫原性ECA的免疫原性的影响，这两种免疫原性ECA分别是去除脂多糖的乙醇可溶制剂和来自R突变型大肠杆菌O14的LPS偶联形式。通过血凝试验测定静脉免疫后的抗体反应。结果表明，PF消除了前者的免疫原性，但没有消除后者的免疫原性。从嗜麦芽窄食单胞菌菌株获得的PF产生了相同的结果。1型和6型铜绿假单胞菌抗血清可中和任一类型产生的PF。这些结果表明，PF改变了ECA的脂质部分而非半抗原决定簇，并且这种活性可被1型或6型铜绿假单胞菌抗血清中和。这一解释与将PF鉴定为脂肪酶是相符的。
