Gannon P J, Jacobs D M, Marx A, Mayer H, Romanowska E, Neter E
Infect Immun. 1982 Jan;35(1):193-201. doi: 10.1128/iai.35.1.193-201.1982.
The immunological activities of three purified preparations of enterobacterial common antigen (ECA) obtained by different procedures were studied. ECA-Ma (method of A. Marx) was from Salmonella typhimurium TV149 (Ra mutant), ECA-My (method of H. Mayer) was from S. montevideo, and ECA-Ro (method of E. Romanowska) was from Shigella sonnei phase I. These preparations, on a weight basis, neutralized similar amounts of ECA antibodies, indicating that the serological activities were comparable. Neither ECA-My nor ECA-Ro elicited specific delayed-type hypersensitivity skin reactions at 24 or 48 h in immunized guinea pigs. ECA-Ma, as well as the nonpurified preparations of the antigens used for immunization, elicited reactions at 24 h but not at 48 h. Thus, ECA-specific delayed-type hypersensitivity was not detected in immunized guinea pigs. Striking differences were noted in the immunogenicity of these antigens, ECA-Ma being highly immunogenic in the rabbit in contrast to ECA-My and ECA-Ro. ECA-Ma was a potent mitogen for guinea pig spleen cells, stimulating high levels of DNA synthesis; ECA-My was only slightly active. The three antigens were mitogenic to spleen cells from both CBA/J and C3H/HeJ mice, although not to the same degree, indicating that this effect is not due to contaminating lipopolysaccharide, since the latter strain of mice is resistant to endotoxin. Since an ECA-Ma extract made from an ECA-negative mutant proved to be mitogenic to murine spleen cells, the mitogenicity is not due to the ECA haptenic determinant. The mitogenic effect is polyclonal in nature, ECA-Ma producing a maximum response on day 3. Thus, the ECA preparations are both B-cell mitogens and polyclonal activators in murine spleen cells. From these studies it is evident that the biological and immunological activities of these purified antigens depend not only on the haptenic determinant but also on associated or bound components of the preparations.
研究了通过不同程序获得的三种纯化肠杆菌共同抗原（ECA）制剂的免疫活性。ECA-Ma（A. Marx方法）来自鼠伤寒沙门氏菌TV149（Ra突变体），ECA-My（H. Mayer方法）来自蒙得维的亚沙门氏菌，ECA-Ro（E. Romanowska方法）来自宋内志贺氏菌I相。以重量计，这些制剂中和了相似量的ECA抗体，表明其血清学活性相当。在免疫的豚鼠中，ECA-My和ECA-Ro在24或48小时均未引发特异性迟发型超敏皮肤反应。ECA-Ma以及用于免疫的未纯化抗原制剂在24小时引发反应，但在48小时未引发反应。因此，在免疫的豚鼠中未检测到ECA特异性迟发型超敏反应。这些抗原的免疫原性存在显著差异，与ECA-My和ECA-Ro相比，ECA-Ma在兔中具有高度免疫原性。ECA-Ma是豚鼠脾细胞的有效促有丝分裂原，可刺激高水平的DNA合成；ECA-My活性较弱。这三种抗原对CBA/J和C3H/HeJ小鼠的脾细胞均有促有丝分裂作用，尽管程度不同，这表明这种作用不是由于污染的脂多糖引起的，因为后一种小鼠品系对内毒素具有抗性。由于从ECA阴性突变体制备的ECA-Ma提取物对鼠脾细胞具有促有丝分裂作用，因此促有丝分裂作用不是由于ECA半抗原决定簇引起的。促有丝分裂作用本质上是多克隆的，ECA-Ma在第3天产生最大反应。因此，ECA制剂在鼠脾细胞中既是B细胞促有丝分裂原又是多克隆激活剂。从这些研究中可以明显看出，这些纯化抗原的生物学和免疫活性不仅取决于半抗原决定簇，还取决于制剂中的相关或结合成分。
