Tong B D, Goldwasser E
J Biol Chem. 1981 Dec 25;256(24):12666-72.
The effect of erythropoietin on the synthesis of the proteins characteristic of mature erythrocyte membranes was studied in cultures of bone marrow cells from adult, polycythemic rats. Stimulated synthesis of the major glycoprotein, glycophorin, was maximal at 30 h and fell to control level by 66 h. Stimulated synthesis of the major integral membrane protein, band 3, occurred at about 18 h was maximal at 66 h, and fell to control level by 114 h. In contrast, stimulated hemoglobin synthesis did not start until after 24 h, was maximal at 96 h, and was at control level at 114 h. Erythropoietin had, in addition, a transient effect on the synthesis of some membrane proteins found in marrow cells but not in mature red cells.
在成年多血质大鼠的骨髓细胞培养物中，研究了促红细胞生成素对成熟红细胞膜特征性蛋白质合成的影响。主要糖蛋白血型糖蛋白的合成在30小时时受到刺激达到最大值，到66小时时降至对照水平。主要整合膜蛋白带3的合成在约18小时开始，在66小时时达到最大值，到114小时时降至对照水平。相比之下，血红蛋白合成的刺激直到24小时后才开始，在96小时时达到最大值，在114小时时处于对照水平。此外，促红细胞生成素对骨髓细胞中发现但成熟红细胞中未发现的一些膜蛋白的合成有短暂影响。
