聚乙烯管中的超速离心法：一种在微升规模上浓缩蛋白质溶液的有效方法。
Ultracentrifugation in polyethylene tubing: an efficient method to concentrate protein solutions on the microliter scale.
作者信息
Biber A, Hempel K
出版信息
J Biochem Biophys Methods. 1981 Oct;5(4):237-9. doi: 10.1016/0165-022x(81)90048-8.
PMID:7310046
Abstract
An ultracentrifugation procedure is described to concentrate protein solutions on the microliter scale. Protein solutions were centrifuged in U-shaped lengths of polyethylene tubing at 160000 X g for 15 h and thereafter fractionated by cutting the tubing. The method can be performed in a conventional ultracentrifuge and needs no special equipment.
摘要
描述了一种用于在微升规模上浓缩蛋白质溶液的超速离心程序。将蛋白质溶液在U形聚乙烯管中以160000×g离心15小时，然后通过切割管子进行分级分离。该方法可以在传统的超速离心机中进行，不需要特殊设备。
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