一种使用葡聚糖凝胶G-25在小体积中高效浓缩蛋白质且回收率高的快速方法。
A rapid method of concentrating proteins in small volumes with high recovery using Sephadex G-25.
作者信息
Saul A, Don M
出版信息
Anal Biochem. 1984 May 1;138(2):451-3. doi: 10.1016/0003-2697(84)90838-8.
PMID:6204553
Abstract
A method for concentrating small (10 to 500 microliter) volumes of protein solutions by mixing in a microcentrifuge tube with measured volumes of dry Sephadex G-25 is described. The tube is centrifuged, and the protein solution concentrated up to fivefold passes through a small hole in the base of the tube and is collected. Recovery of protein is 90-95%.
摘要
描述了一种通过在微量离心管中与定量的干燥葡聚糖凝胶G - 25混合来浓缩少量（10至500微升）蛋白质溶液的方法。将管子离心，浓缩至五倍体积的蛋白质溶液通过管底部的一个小孔并被收集。蛋白质回收率为90 - 95%。
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