Harff G A, van Leeuwen C
J Clin Chem Clin Biochem. 1981 Oct;19(10):1027-31. doi: 10.1515/cclm.1981.19.10.1027.
We report the evaluation of a novel clinical chemical analyser, the OLLI CD, which is highly automated compared to its predecessor, the OLLI 3000 analyser. The precision of the dispensing unit, the OLLI D, was assessed with the aid of aqueous solutions of 131I. Sample pipetting of 10 microliter resulted in a coefficient of variation of 0.81%. Lower volumes were unreliable. However, precision of the addition of start reagent for kinetic methods appeared to be only 2.7% for 20 microliter, 1.7% for 50 microliter and 2.1% for 250 microliter. The linearity of response and the precision of the photometer unit, the OLLI C, was assessed with cobalt(II)sulfate solutions. The linearity ranged up to an absorbance of 2.5. The reproducibility of the colorimeter ranged from A510nm 0.0011 to 0.0047. With this analyser good correlations were obtained for alanine aminotransferase (EC 2.6.1.2) vs LKB 8600 reaction rate analyser; for creatinine vs Mark II Autoanalyzer; and for total protein vs SMA 6/60 Autoanalyzer. These three determinations showed acceptable between-day variations. General instrument performance was acceptable.
我们报告了一种新型临床化学分析仪OLLI CD的评估情况,与它的前身OLLI 3000分析仪相比,该分析仪具有更高的自动化程度。借助¹³¹I水溶液对OLLI D加样单元的精密度进行了评估。吸取10微升样品时,变异系数为0.81%。吸取更低体积的样品时结果不可靠。然而,对于动力学方法,加入起始试剂的精密度在吸取20微升时为2.7%,50微升时为1.7%,250微升时为2.1%。用硫酸钴溶液评估了OLLI C光度计单元的响应线性和精密度。线性范围高达吸光度2.5。比色计的重现性在A510nm为0.0011至0.0047之间。使用该分析仪,丙氨酸氨基转移酶(EC 2.6.1.2)与LKB 8600反应速率分析仪之间、肌酐与Mark II自动分析仪之间以及总蛋白与SMA 6/60自动分析仪之间均获得了良好的相关性。这三项测定显示出可接受的日间差异。仪器的总体性能是可接受的。