用草酸氧化酶直接分光光度法测定血清和尿液中的草酸盐。

Direct spectrophotometric determination of serum and urinary oxalate with oxalate oxidase.

作者信息

Kohlbecker G, Butz M

出版信息

J Clin Chem Clin Biochem. 1981 Nov;19(11):1103-6. doi: 10.1515/cclm.1981.19.11.1103.

DOI:10.1515/cclm.1981.19.11.1103

Abstract

A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase. Percentage recovery of added oxalate was 99 +/- 4 in serum, and 98 +/- 4 in urine (n - 10). Oxalate serum levels varied from 16.9 to 44.8 mumol/l. Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples. The detection limit is 5 mumol/l.

摘要

描述了一种使用草酸氧化酶直接光度法测定血清和尿液中草酸盐的新酶法。生成的过氧化氢用过氧化氢酶和醛脱氢酶的偶联酶系统进行测定。血清中添加草酸盐的回收率为99±4%，尿液中为98±4%（n = 10）。血清草酸盐水平在16.9至44.8μmol/l之间。无需对样品进行耗时的预处理，20分钟内即可测定草酸盐值。检测限为5μmol/l。