Hönow R, Bongartz D, Hesse A
Klinik und Poliklinik für Urologie, Universität Bonn, Germany.
Clin Chim Acta. 1997 May 28;261(2):131-9. doi: 10.1016/s0009-8981(97)06521-2.
In this paper we present an improved method for the selective and sensitive determination of oxalate in different matrices such as urine, plasma, and food. The method uses ion chromatography for the separation of anions. To overcome problems with interfering matrix-anions, colourings, and macromolecules, we used an inline enzyme-reactor (ER) containing immobilised oxalate oxidase, which converts oxalate to hydrogen peroxide. Hydrogen peroxide was analysed with high sensitivity by amperometric detection. The determination limit for the HPLC-ER method was 1.5 mumol/1, the mean recovery in urine was 102%. The evaluation in a urinary matrix achieved C.V. values from 2.2% to 6.7% for the within-run precision and C.V. values from 3.7% to 8.6% for the between-batch precision. The results of the new method were statistically equivalent to those obtained by enzymatic kits. We present first results of the HPLC-ER method, when applied to body fluids and food analysis.
在本文中，我们提出了一种改进的方法，用于选择性和灵敏地测定不同基质（如尿液、血浆和食物）中的草酸盐。该方法采用离子色谱法分离阴离子。为克服干扰性基质阴离子、色素和大分子带来的问题，我们使用了一种包含固定化草酸氧化酶的在线酶反应器（ER），该酶将草酸盐转化为过氧化氢。通过安培检测对过氧化氢进行高灵敏度分析。HPLC-ER方法的测定限为1.5 μmol/L，尿液中的平均回收率为102%。在尿液基质中的评估结果显示，批内精密度的变异系数（C.V.）值为2.2%至6.7%，批间精密度的C.V.值为3.7%至8.6%。新方法的结果与酶试剂盒获得的结果在统计学上等效。我们展示了HPLC-ER方法应用于体液和食物分析时的初步结果。
