Mautner H G, Merrill R E, Currier S F, Harvey G
J Med Chem. 1981 Dec;24(12):1534-7. doi: 10.1021/jm00144a035.
The interaction of a series of aromatic dyes with the coenzyme A binding site of choline acetyltransferase was studied. Several of the dyes were very potent inhibitors of the enzyme. With few exceptions, inhibition was competitive with respect to acetylcoenzyme A and noncompetitive with respect to choline. It appears likely that inhibition by dyes such as Reactive Blue 2 (Cibacron Blue F3GA) or Congo Red, as in the case of coenzyme A interactions, involves hydrophobic bonding, as well as a coulombic interaction with an arginine residue.
研究了一系列芳香族染料与胆碱乙酰转移酶辅酶A结合位点的相互作用。其中几种染料是该酶的强效抑制剂。除少数例外，抑制作用对乙酰辅酶A而言是竞争性的，对胆碱而言是非竞争性的。与辅酶A相互作用的情况类似，活性蓝2（汽巴克隆蓝F3GA）或刚果红等染料的抑制作用似乎涉及疏水键合以及与精氨酸残基的库仑相互作用。
