乙酰辅酶A和辅酶A类似物。它们对大鼠脑胆碱乙酰转移酶的影响。
Acetyl-coenzyme A and coenzyme A analogues. Their effects on rat brain choline acetyltransferase.
作者信息
Rossier J
出版信息
Biochem J. 1977 Aug 1;165(2):321-6. doi: 10.1042/bj1650321.
Abstract
Choline acetyltransferase has the same affinity for acetyl-CoA, propionyl-CoA and butyryl-CoA (Km=1.4 micron). Choline acetyltransferase may use the two latter compounds as substrate, but the longer the acyl chain the lower will be Vmax. CoA is an inhibitor (Ki=1.8 micron). The position of the 3'-phosphate is of primary importance. Desphospho-CoA is a weak inhibitor (Ki=500 micron). 5'-AMP is already an inhibitor (Ki=2500 micron). Phosphopantetheine is not an inhibitor. Dextran Blue is a potent inhibitor (Ki=0.05 micron). Choline acetyltransferase binds to hydrophobic affinity columns. Because of its affinity for nucleotides, affinity for Dextran Blue and hydrophobicity, it is proposed that it contains the 'nucleotide fold', which is a common structural domain present in several enzymes binding nucleotides.
摘要
胆碱乙酰转移酶对乙酰辅酶A、丙酰辅酶A和丁酰辅酶A具有相同的亲和力（米氏常数Km = 1.4微米）。胆碱乙酰转移酶可能将后两种化合物用作底物，但酰基链越长，最大反应速度（Vmax）就越低。辅酶A是一种抑制剂（抑制常数Ki = 1.8微米）。3'-磷酸的位置至关重要。去磷酸辅酶A是一种弱抑制剂（Ki = 500微米）。5'-腺苷酸已经是一种抑制剂（Ki = 2500微米）。磷酸泛酰巯基乙胺不是抑制剂。葡聚糖蓝是一种强效抑制剂（Ki = 0.05微米）。胆碱乙酰转移酶能与疏水亲和柱结合。由于它对核苷酸有亲和力、对葡聚糖蓝有亲和力且具有疏水性，因此有人提出它含有“核苷酸折叠”结构，这是存在于几种结合核苷酸的酶中的一种常见结构域。
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本文引用的文献
1
THE SYNTHESIS OF SELENOCOENZYME A.硒辅酶A的合成
J Am Chem Soc. 1965 Jun 20;87:2708-16. doi: 10.1021/ja01090a031.
2
The acyl-group specificity of choline acetylase.胆碱乙酰转移酶的酰基特异性
Biochem J. 1959 Nov;73(3):447-58. doi: 10.1042/bj0730447.
3
Tissue sulfhydryl groups.组织巯基
Arch Biochem Biophys. 1959 May;82(1):70-7. doi: 10.1016/0003-9861(59)90090-6.
4
Properties and biological significance of choline acetylase.胆碱乙酰转移酶的性质及生物学意义。
Yale J Biol Med. 1957 Feb;29(4):403-35.
5
A coenzyme A analogue, desulpho-coA; preparation and effects on various enzymes.一种辅酶A类似物，脱硫辅酶A；制备及其对各种酶的作用。
Biochem Biophys Res Commun. 1966 Apr 19;23(2):208-13. doi: 10.1016/0006-291x(66)90529-8.
6
Halogen substituted derivatives of acetylcholine as inhibitors of choline acetyltransferase.作为胆碱乙酰转移酶抑制剂的乙酰胆碱的卤素取代衍生物。
Life Sci. 1969 May 15;8(10):511-6. doi: 10.1016/0024-3205(69)90249-5.
7
Choline acetyltransferase binding to and release from membranes.胆碱乙酰转移酶与膜的结合及从膜上的释放
Biochem J. 1968 Sep;109(3):389-98. doi: 10.1042/bj1090389.
8
Enzymic acetylation of the stereoisomers of alpha- and beta-methyl choline.α-和β-甲基胆碱立体异构体的酶促乙酰化作用。
Biochem Pharmacol. 1970 Nov;19(11):2925-7. doi: 10.1016/0006-2952(70)90032-8.
9
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J Neurochem. 1970 Feb;17(2):171-7. doi: 10.1111/j.1471-4159.1970.tb02198.x.
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