Ksenzhek O S, Vecherova V V, Serebritskiĭ V M, Nefedov V G
Mikrobiologiia. 1981 Jul-Aug;50(4):645-9.
The main factors limiting the possibility of organizing the cultivation of the hydrogen-oxidizing bacterium Alcaligenes eutropha Z-1 in combination with electrolysis were determined: accumulation of the oxidizing agents (hypochlorite, persulfate ions, hydrogen peroxide) in the process of electrolysis and deposition of microelements contained in the growth medium on the cathode. The limits for the susceptibility of the bacterium to the oxidizing agents were established. The rate of accumulation of the oxidizing agents and formation of the cathode deposit were found to depend on the current density, the pH and composition of a solution, and several other factors. The concentration of hypochlorite decreased if the content of chloride ions in the growth medium was reduced to approximately 10(-5) g/l; the concentrations of persulfate and hydrogen peroxide is decreased using a certain electrolysis regime and adding ferrous ions which reduce the oxidizing agents. The cathode deposit is dissolved by periodically reversing the current in the course of electrolysis.
确定了限制将氢氧化细菌真养产碱杆菌Z-1与电解相结合进行培养的可能性的主要因素:电解过程中氧化剂(次氯酸盐、过硫酸根离子、过氧化氢)的积累以及生长培养基中所含微量元素在阴极上的沉积。确定了该细菌对氧化剂的敏感限度。发现氧化剂的积累速率和阴极沉积物的形成取决于电流密度、溶液的pH值和组成以及其他几个因素。如果将生长培养基中氯离子的含量降低至约10^(-5) g/l,次氯酸盐的浓度会降低;使用特定的电解方式并添加可还原氧化剂的亚铁离子,可降低过硫酸盐和过氧化氢的浓度。在电解过程中通过周期性地反向电流来溶解阴极沉积物。