Hallas B H, Das G D
J Neurol Sci. 1978 Nov;39(1):111-22. doi: 10.1016/0022-510x(78)90192-2.
N-Ethyl-N-nitrosourea (ENU) was administered intravenously to pregnant Wistar-albino rats on days 14--21 of gestation in order to study the teratological effects of the carcinogen on the developing brain. Offspring were killed 60 days postnatally, and the brains examined histologically by cresyl violet staining and Golgi-Cox preparations. Macroscopic examination of the brains revealed a graded pattern of microcephaly. Injection of ENU on day 14 of gestation gave the most severe effects and injection on day 21 the least effects. Microscopic examination of the neocortex revealed a graded reduction in the lateral and sagittal lengths, thickness, and the number of cells in a sample slab of the cortex. In the Golgi-Cox preparations the pyramidal neurons of cortical layers III and V, as well as neurons of other layers, were seen to have fewer secondary and tertiary dendrites, and the length of their dendrites appeared stunted. These characteristics also were graded in relation to the day of injection of ENU. Altered cytology and cytoarchitecture of the neocortex and possible underlying mechanisms are discussed.
在妊娠第14至21天，将N-乙基-N-亚硝基脲（ENU）静脉注射给怀孕的Wistar白化大鼠，以研究这种致癌物对发育中大脑的致畸作用。子代在出生后60天处死，大脑通过甲酚紫染色和高尔基-考克斯染色法进行组织学检查。大脑的宏观检查显示出小头畸形的分级模式。在妊娠第14天注射ENU产生的影响最严重，而在第21天注射产生的影响最小。新皮质的显微镜检查显示，皮质样本板的横向和矢状长度、厚度以及细胞数量呈分级减少。在高尔基-考克斯染色制剂中，可见皮质层III和V的锥体神经元以及其他层的神经元具有较少的二级和三级树突，并且它们的树突长度似乎发育不良。这些特征也与ENU注射日有关而呈分级变化。文中讨论了新皮质细胞学和细胞结构的改变以及可能的潜在机制。
