通过使用双标记的[14C]黄素腺嘌呤二核苷酸和[14C, 32P]黄素单核苷酸研究注射黄素的代谢。

Metabolism of injected flavins studied by using double-labeled [14C]flavin adenine dinucleotide and [14C, 32P]flavin mononucleotide.

作者信息

Okuda J, Nagamine J, Okumura M, Yagi K

出版信息

J Nutr Sci Vitaminol (Tokyo). 1978;24(5):505-10. doi: 10.3177/jnsv.24.505.

DOI:10.3177/jnsv.24.505

Abstract

The metabolism of flavins in mouse was studied with [F-(2)-14C, A-(2,8)-14C]FAD and [F-(2)-14C, 32P]FMN. Ninety minutes after injection, radioactive isoalloxazine nucleus of double-labeled FAD was markedly incorporated into FAD, FMN and riboflavin in the liver, whereas a small amount of radioactive adenine nucleus of double-labeled FAD was found in FAD in the liver. In the case of FMN, radioactive isoalloxazine nucleus of double-labeled FMN was markedly incorporated into FAD, FMN and riboflavin in the liver, whereas only a minute amount of radioactive phosphorus was incorporated into FMN and FAD in the same organ. These results indicate that FMN and FAD injected are rapidly hydrolyzed and resynthesized in animal body.

摘要

用[F-(2)-14C, A-(2,8)-14C]FAD和[F-(2)-14C, 32P]FMN研究了黄素在小鼠体内的代谢。注射后90分钟，双标记FAD的放射性异咯嗪核显著掺入肝脏中的FAD、FMN和核黄素中，而在肝脏中的FAD中发现了少量双标记FAD的放射性腺嘌呤核。就FMN而言，双标记FMN的放射性异咯嗪核显著掺入肝脏中的FAD、FMN和核黄素中，而在同一器官中只有微量放射性磷掺入FMN和FAD中。这些结果表明，注射的FMN和FAD在动物体内迅速水解并重新合成。