紫外线照射维生素D缺乏大鼠血液和肝脏中25-羟基维生素D3的鉴定与测定
Identification and determination of 25-hydroxyvitamin D3 in the blood and liver of vitamin D-deficient rats irradiated with ultraviolet light.
作者信息
Okano T, Mizuno K, Kobayashi T
出版信息
J Nutr Sci Vitaminol (Tokyo). 1978;24(5):511-8. doi: 10.3177/jnsv.24.511.
The intestinal calcium transport activity and serum calcium and phosphorous concentrations of vitamin D-deficient rats were increased by irradiation with an ultraviolet (UV) lamp. The existence of 25-hydroxyvitamin D3(25-OH-D3) in their bloods and livers was physicochemically confirmed by high-performance liquid chromatography (HPLC), gas-liquid chromatography (GLC) and mass fragmentography, whereas the compound could not be detected in the tissues of non-irradiated rats. The results strongly suggested that vitamin D3 in vivo generated in irradiated rat skin might be normally metabolized and utilized to prevent rickets. The level of 25-OH-D3 in the tissues was determined by a HPLC method.
用紫外线灯照射可提高维生素D缺乏大鼠的肠道钙转运活性以及血清钙和磷浓度。通过高效液相色谱法（HPLC）、气液色谱法（GLC）和质谱碎片分析法从物理化学角度证实了其血液和肝脏中存在25-羟基维生素D3（25-OH-D3），而在未照射大鼠的组织中未检测到该化合物。结果有力地表明，经照射的大鼠皮肤中体内生成的维生素D3可能正常代谢并用于预防佝偻病。组织中25-OH-D3的水平通过HPLC法测定。
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