Rogawski M A, Goodrich J T, Gershon M D, Touloukian R J
J Pediatr Surg. 1978 Dec;13(6D):608-15. doi: 10.1016/s0022-3468(78)80102-x.
The distribution of enteric serotonergic neurons was studied in patients with Hirschsprung's disease. Specimens of bowel obtained at surgery were incubated in vitro with tritiated serotonin (3H-5-HT) in the presence of a high concentration of nonradioactive norepinephrine. Sites of high-affinity 3H-5-HT uptake were visualized by light-microscopic autoradiography. Specimens taken from ganglionic regions of the intestine (distal ileum or colon) showed intense labeling of the neuropil within the myenteric plexus. Silver grains were localized in a pericellular distribution around ganglion cells, but the ganglion cells themselves were relatively free of overlying silver grains. Corresponding regions of aganglionic colon or rectum demonstrated silver grain densities equivalent to background levels. Specific labeling was absent over the large nerve trunks in this region. These results suggest that 5-HT-containing neurons are present in the normal human intestine and that these neurons are absent in the aganglionic segment in Hirshsprung's disease.
研究了先天性巨结肠症患者肠道中5-羟色胺能神经元的分布情况。手术获取的肠标本在体外与高浓度非放射性去甲肾上腺素存在下的氚标记5-羟色胺（3H-5-HT）一起孵育。通过光学显微镜放射自显影观察高亲和力3H-5-HT摄取位点。取自肠神经节区域（回肠末端或结肠）的标本显示，肌间神经丛内的神经毡有强烈标记。银颗粒呈细胞周围分布于神经节细胞周围，但神经节细胞本身相对没有覆盖其上的银颗粒。无神经节的结肠或直肠的相应区域显示银颗粒密度与背景水平相当。该区域的大神经干上没有特异性标记。这些结果表明，正常人体肠道中存在含5-羟色胺的神经元，而在先天性巨结肠症的无神经节段中这些神经元不存在。
