Szabolcsi G, Cseke E
Acta Biol Med Ger. 1981;40(4-5):471-7.
The membrane of swollen erythrocytes was found to be more resistant to hypotonicity at 37 degrees C than at 0 degrees C either in the presence or absence of added glucose and even more resistant if ATP formation was stimulated by preincubation of the sedimented cells with adenosine. The membrane, irrespective of temperature and metabolism behaves as a molecular sieve for proteins, i.e. the smaller the molecular radius of a protein, the higher is the amount released by molecular sieving. This phenomenon allows one to discriminate between uniform distribution of proteins within the cell and complex formation of enzymes. Some glycolytic enzymes catalyzing reactions between triosephosphate and lactate production were found to be clustered near the surface of the cell but not bound to the membrane. The data indicate that some of these enzymes may form complexes under in vivo conditions.
无论是在添加葡萄糖与否的情况下，肿胀红细胞的膜在37℃时比在0℃时更耐低渗；如果通过用腺苷预孵育沉淀细胞来刺激ATP形成，其耐低渗性更强。无论温度和代谢如何，细胞膜对蛋白质起着分子筛的作用，即蛋白质的分子半径越小，通过分子筛作用释放的量就越高。这种现象使得人们能够区分细胞内蛋白质的均匀分布和酶的复合物形成。一些催化磷酸丙糖和乳酸生成之间反应的糖酵解酶被发现聚集在细胞表面附近，但不与膜结合。数据表明，其中一些酶在体内条件下可能形成复合物。
