不同介质中人类红细胞的能量与热量产生
Energy and heat production of human erythrocytes in different media.
作者信息
de Verdier C H
出版信息
Acta Biol Med Ger. 1981;40(4-5):699-702.
Variation in ATP consumption of human erythrocytes was achieved by incubation in different media. Addition of ouabain to a conventional isotonic medium or elimination of the ionic Na+-K+ gradient across the membrane decreases lactate production of the cells by about 40%. Applying a calcium-gradient of 1 mmol . l-1 across the membrane increased glycolysis by about 25%. The various introduced changes in the medium and thus in ATP-consumption cause similar alterations in heat production and lactate production. The experimental results give no suggestions explaining the origin of the part of the heat production that is unrelated to glycolysis.
通过在不同培养基中孵育实现了人类红细胞ATP消耗的变化。向传统等渗培养基中添加哇巴因或消除跨膜的离子Na⁺-K⁺梯度可使细胞的乳酸生成减少约40%。施加1 mmol·l⁻¹的跨膜钙梯度可使糖酵解增加约25%。培养基中引入的各种变化以及由此导致的ATP消耗变化会引起产热和乳酸生成的类似改变。实验结果并未给出解释与糖酵解无关的那部分产热来源的线索。
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