Gundersen S, Jacobsen J, Funderud S
Acta Pathol Microbiol Scand C. 1981 Oct;89(5):329-32. doi: 10.1111/j.1699-0463.1981.tb02708.x.
Inhibition of cell growth by B6D2F1 (C57Bl/6 X DBA/2) mouse serum in tissue culture wells confirming previous findings in the soft agar tumour colony assay is reported. The assay was time saving, more sensitive, and the amount of mouse serum used could in each experiment be reduced by 95%. In addition non-neoplastic cells ordinarily not forming colonies in the soft agar assay could be tested. Such cells were less sensitive to the growth inhibitory effect when compared to neoplastic cells.
据报道，B6D2F1（C57Bl/6×DBA/2）小鼠血清在组织培养孔中对细胞生长具有抑制作用，这证实了先前在软琼脂肿瘤集落试验中的发现。该试验节省时间，更灵敏，并且每次实验中所用小鼠血清的量可减少95%。此外，通常在软琼脂试验中不形成集落的非肿瘤细胞也可以进行检测。与肿瘤细胞相比，这类细胞对生长抑制作用不太敏感。
