Gundersen S, Wibe E, Funderud S, Grzenlak-Puczyinska I, Godal T
Acta Pathol Microbiol Scand C. 1981 Aug;89(4):223-8. doi: 10.1111/j.1699-0463.1981.tb02691.x.
Differences in tumour susceptibility between strains of mice (C57Bl/6 and C57Bl/6 X DBA/2 = B6D2F1) could be demonstrated for several tumours of C57Bl origin, both solid tumours (B16 melanoma and Lewis lung carcinoma) and lymphomas (RBL-5, 136-3 and ALC). Serum from mice with high tumour resistance in vivo (B6D2F1) showed an inhibitory effect on tumour colony formation in a soft agar colony assay. Serum from mice with lower tumour resistance (C57Bl/6) had no effect. When other F1 hybrids of C57Bl/6 parental origin were tested, the same correlation between in vitro inhibition of tumour colony formation and in vivo susceptibility was found. The serum factor was species non-specific, since the activity was expressed against in vitro grown cell lines of human origin. The tumour colony inhibitory activity was heat sensitive (56 degrees C for 30 minutes), precipitable by (NH4)2SO4, and not removed by adsorption on tumour cells. These results demonstrate the existance of a naturally-occurring humoral tumerostatic factor(s) which correlates to in vivo susceptibility to tumour cells. Its relationship to NK cell activity is discussed.
对于源自C57Bl的几种肿瘤，包括实体瘤（B16黑色素瘤和Lewis肺癌）和淋巴瘤（RBL-5、136-3和ALC），可以证明小鼠品系（C57Bl/6和C57Bl/6×DBA/2 = B6D2F1）之间的肿瘤易感性存在差异。体内具有高肿瘤抗性的小鼠（B6D2F1）的血清在软琼脂集落试验中对肿瘤集落形成显示出抑制作用。肿瘤抗性较低的小鼠（C57Bl/6）的血清则没有作用。当测试其他源自C57Bl/6亲本品系的F1杂种时，在体外对肿瘤集落形成的抑制与体内易感性之间发现了相同的相关性。血清因子具有种属非特异性，因为其活性针对源自人的体外培养细胞系表现出来。肿瘤集落抑制活性对热敏感（56℃，30分钟），可被硫酸铵沉淀，并且不会因吸附在肿瘤细胞上而去除。这些结果证明存在一种天然存在的体液肿瘤抑制因子，其与体内对肿瘤细胞的易感性相关。文中还讨论了它与自然杀伤细胞活性的关系。
