Ekström J, Uvelius B
Acta Physiol Scand. 1981 Aug;112(4):443-7. doi: 10.1111/j.1748-1716.1981.tb06842.x.
Urinary bladders of rats were denervated by bilateral excision of the pelvic ganglion and removed 10 days after the operation. They were filled with 0.75 ml saline and a longitudinal muscle strip was marked out, measured and dissected out. Strips from normal bladders filled with the same volume were used as controls. Denervated bladders were 4-5 times heavier than control bladders. Muscle strips from denervated bladders showed, in contrast to controls, marked phasic spontaneous contractions which were unaffected by tetrodotoxin, indicating a myogenic origin. Active tension in response to AC stimulation was measured at different lengths. In relation to the in situ length (Lin situ) at 0.75 ml the denervated strips had to be stretched to much greater extent than controls in order to reach optimum length (L0) for force development. Furthermore, the denervated strips shortened less in relation to Lin situ than the controls. If active length-tension relations were expressed in relation to L0, the difference between denervated and control strips was abolished. Maximal active force was the same for denervated and control strips. Water content increased significantly in denervated bladders. The results suggest a remodelling of the smooth muscle structure in denervated bladders; the characteristics of the contractile machinery seem, however, to be unaltered.
通过双侧切除盆腔神经节使大鼠膀胱去神经支配，并在手术后10天取出。向膀胱内注入0.75 ml生理盐水，划出一条纵行肌条，测量并分离出来。将注入相同体积生理盐水的正常膀胱的肌条用作对照。去神经支配的膀胱比对照膀胱重4 - 5倍。与对照相比，去神经支配膀胱的肌条显示出明显的阶段性自发收缩，且不受河豚毒素影响，表明其起源于肌源性。在不同长度下测量对交流电刺激的主动张力。相对于在注入0.75 ml生理盐水时的原位长度（Lin situ），去神经支配的肌条必须比对照肌条拉伸更大程度才能达到产生力量的最佳长度（L0）。此外，相对于Lin situ，去神经支配的肌条缩短程度比对照肌条小。如果以L0表示主动长度 - 张力关系，则去神经支配的肌条与对照肌条之间的差异消失。去神经支配的肌条和对照肌条的最大主动力相同。去神经支配的膀胱含水量显著增加。结果表明去神经支配的膀胱平滑肌结构发生了重塑；然而，收缩机制的特性似乎未改变。
